Centre for Health Psychology

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Browsing Centre for Health Psychology by Author "Baburao, Nimala"
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    Changes in protein composition and protein phosphorylation during somatic embryogenesis and plant regeneration in peanut (Arachis hypogaea L.)
    ( 2009-08-04) Roja Rani, A. ; Venkateswarlu, P. ; Padmaja, G. ; Venkatesh, K. ; Baburao, Nimala
    Changes in protein profiles and protein phosphorylation were studied in various stages of germinating somatic and zygotic embryos. Many proteins, which were expressed in cotyledonary stage somatic embryos, were also present in the zygotic embryos obtained from mature dry seed. The intensity of 22 kDa protein was much higher in zygotic embryos in comparison to cotyledonary somatic embryos. Proteins of 55, 53, 27 and 25 kDa did not vary in expression during different stages of germination of somatic and zygotic embryos. There was rapid accumulation of 28 kda protein in germinating zygotic and somatic embryos at 3rd stage and persisted during the subsequent stages of germination indicating the emergence of radical and plumule. A clear difference was observed in the patterns of protein phosphorylation in germinating somatic and zygotic embryos. A 50 kDa protein was heavily phosphorylated in mature zygotic embryos at 2nd stage of germination and disappeared in 3rd stage of germination. Protiens of 50 and 43 kDa proteins showed enhanced phosphorylation up to 3rd stage and drastic reduction was observed at 4th stage. In addition, phosphorylation of 68 and 65 kDa specifically appeared in 3rd stage of germinating somatic embryos and were not observed during any germinating stages of zygotic embryos. © 2009 Academic Journals.
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    Polypeptides associated with the induction and maturation of direct somatic embryogenesis in peanut (Arachis hypogaea L.)
    ( 2009-08-04) Roja Rani, A. ; Venkateswarlu, P. ; Kumar, Arun ; Padmaja, G. ; Baburao, Nimala
    Analysis of protein patterns in mature zygotic embryo axes and somatic embryos by two-dimensional gel electrophoresis revealed that protein of spot no. E4 (37 kDa, pI 9.5) and E5 (37 kDa, pI 9.8) are present only in the 6 day old culture indicating their importance for induction of somatic embryogenesis. Proteins of spot no. E1 (53kDa, pI 8.2), E2 (53kDa, pI 8.3) and E3 (37 kDa, pI 9.3) expressed in 6 day-old immature zygotic embryo axis cultures were also present in globular staged somatic embryos and these proteins are possibly associated with early stages of somatic embryogenesis. Proteins of 65 kDa separated into different spots of C1, C2, C3, C4 with pI values ranging from 6.3-9.6 on two-dimensional gel are present only in zygotic embryos. Proteins of C2 (65 kDa, pI 7.5), C8 (22 kDa, pI 7.8), C 10 (20 kDa, pI 5.3) C11 (20 kDa, pI 5.5) were specifically expressed in mature zygotic embryo axes and are possibly associated with storage or late embryonic proteins. A few proteins showed differential expression during various stages of somatic embryos and many proteins that expressed in cotyledonary somatic embryos and were also present in immature and mature zygotic embryo axes. © 2009 Academic Journals.