Computational Biology - Publications
Permanent URI for this collection
Browse
Browsing Computational Biology - Publications by Author "Achary, M. S."
Results Per Page
Sort Options
-
ItemDesign, synthesis, and evaluation of mixed imine-amine pyrrolobenzodiazepine dimers with efficient DNA binding affinity and potent cytotoxicity( 2004-10-15) Kamal, Ahmed ; Ramesh, G. ; Srinivas, O. ; Ramulu, P. ; Laxman, N. ; Rehana, Tasneem ; Deepak, M. ; Achary, M. S. ; Nagarajaram, H. A.Synthesis of mixed imine-amine pyrrolobenzodiazepine (PBD) dimers that are comprised of DC-81 and secondary amine (N10) of DC-81 subunits tethered to their C8 positions through alkanedioxy linkers (comprised of three and five carbons) is described. These noncross-linking unsymmetrical molecules exhibit significant DNA minor groove binding ability and one of them 5b linked through the pentanedioxy chain exhibits efficient DNA binding ability (ΔT m = 11.0°C) when compared to naturally occurring DC-81, 1 (ΔT m = 0.7°C). The imine-amine PBD dimers exhibit promising in vitro antitumor activity in a number of human cancer cell lines. © 2004 Elsevier Ltd. All rights reserved.
-
ItemProfilin oligomerization and its effect on poly (l-proline) binding and phosphorylation( 2009-10-01) Korupolu, Radhika V. ; Achary, M. S. ; Aneesa, F. ; Sathish, K. ; Wasia, R. ; Sairam, M. ; Nagarajaram, H. A. ; Singh, Surya S.Profilin is a cytoskeletal protein that interacts specifically with actin, phosphoinositides and poly (l-proline). Experimental results and in silico studies revealed that profilin exists as dimer and tetramer. Profilin oligomers possess weak affinity to poly (l-proline) due to unavailability of binding sites in dimers and tetramers. Phosphorylation studies indicate that profilin dimers are not phosphorylated while teramers are preferentially phosphorylated over monomers. In silico studies revealed that PKC phosphorylation site, S137 is buried in dimer while it is accessible in tetramer. © 2009 Elsevier B.V. All rights reserved.