Application of fast chlorophyll a fluorescence transient (OJIP) analysis to monitor functional integrity of pea (Pisum sativum) mesophyll protoplasts during isolation

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dc.contributor.author Sunil, B.
dc.contributor.author Riazunnisa, K.
dc.contributor.author Sai Krishna, T.
dc.contributor.author Schansker, Gert
dc.contributor.author Strasser, Reto J.
dc.contributor.author Raghavendra, Agepati S.
dc.contributor.author Mohanty, Prasanna
dc.date.accessioned 2022-03-27T03:50:57Z
dc.date.available 2022-03-27T03:50:57Z
dc.date.issued 2008-02-01
dc.description.abstract Intact and metabolically very active mesophyll protoplasts were isolated rapidly from pea (Pisum sativum) leaves. The functional performance of protoplasts at various stages of their isolation was analyzed by using fast Chl a fluorescence OJIP transients and compared with that of intact leaves. The results demonstrated that the OJIP transients could successfully be used to monitor the quality of mesophyll protoplasts at different isolation steps. The protoplasts maintained their integrity and photosynthetic status very well, and their performance was very similar to that of the intact leaves.
dc.identifier.citation Indian Journal of Biochemistry and Biophysics. v.45(1)
dc.identifier.issn 03011208
dc.identifier.uri https://dspace.uohyd.ac.in/handle/1/5747
dc.subject Chl a fluorescence
dc.subject Functional integrity
dc.subject Mesophyll protoplasts
dc.subject O-J-I-P transients
dc.subject Pisum sativum
dc.subject Protoplast isolation
dc.title Application of fast chlorophyll a fluorescence transient (OJIP) analysis to monitor functional integrity of pea (Pisum sativum) mesophyll protoplasts during isolation
dc.type Journal. Article
dspace.entity.type
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