Mycobacterium oryzae sp. Nov., a scotochromogenic, rapidly growing species is able to infect human macrophage cell line

Abstract

Gram-stain-positive, acid-fast-positive, rapidly growing, rod-shaped bacteria (designated as strains JC290T, JC430 and JC431) were isolated from paddy cultivated soils on the Western Ghats of India. Phylogenetic analysis placed the three strains among the rapidly growing mycobacteria, being most closely related to Mycobacterium tokaiense 47503T(98.8% 16S rRNA gene sequence similarity), Mycobacterium murale MA112/96T(98.8 %) and a few other Mycobacterium species. The level of DNA–DNA reassociation of the three strains with M. tokaiense DSM 44635Twas 23.4±4% (26.1±3 %, reciprocal analysis) and 21.4±2% (22.1 ±4 %, reciprocal analysis). The three novel strains shared > 99.9% 16S rRNA gene sequence similarity and DNA–DNA reassociation values > 85 %. Furthermore, phylogenetic analysis based on concatenated sequences (3071 bp) of four housekeeping genes (16S rRNA, hsp65, rpoB and sodA) revealed that strain JC290T is clearly distinct from all other Mycobacterium species. The three strains had diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositolmannosides, unidentified phospholipids, unidentified glycolipids and an unidentified lipid as polar lipids. The predominant isoprenoid quinone for all three strains was MK-9(H2). Fatty acids were C17:1ω7c, C16:0, C18:1ω9c, C16:1ω7c/C16:1ω6c and C19:1ω7c/C19:1ω6c for all the three strains. On the basis of phenotypic, chemotaxonomic and phylogenetic data, it was concluded that strains JC290T, JC430 and JC431 are members of a novel species within the genus Mycobacterium and for which the name Mycobacterium oryzae sp. nov. is proposed. The type strain is JC290T(=KCTC 39560T=LMG 28809T).