Chryseomicrobium amylolyticum sp. nov., isolated from a semi-arid tropical soil, and emended descriptions of the genus Chryseomicrobium and Chryseomicrobium imtechense

Abstract

A rod-shaped, non-motile, Gram-stain-positive, catalase-positive, starch-hydrolysing strain, JC16T, was isolated from a semi-arid tropical soil from India. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain JC16T clustered with the type species of the genus Chryseomicrobium, Chryseomicrobium imtechense MW 10T, a member of the family Planococcaceae within the phylum Firmicutes with 99.3 % sequence similarity. Major ( > 10 %) fatty acids of strain JC16T were iso-C15: 0 and iso-C16: 0. Minor ( < 10 and > 1 %) amounts of C16: 0, iso-C14: 0, anteiso-C15: 0, iso-C17: 0, anteiso-C17: 0, iso-C17: 1ω10c and C16: 1ω11c are present in strain JC16T. Polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phospholipids (PL2-4), aminolipids (AL1, 2) and an unknown lipid. Cell wall peptidoglycan was of the type L-Orn-D-Glu. The quinone system was composed of MK-7, MK-8 and MK-6. Genomic DNA G+C content of strain JC16T was 57.6 mol%. Distinct physiological, chemotaxonomic and genotypic differences (37 % reassociation based on DNA-DNA hybridization) from Chryseomicrobium imtechense MW 10T support the classification of strain JC16T as a representative of a novel species in the genus Chryseomicobium, for which the name Chryseomicrobium amylolyticum sp. nov. (type strain JC16T = DSM 23442T = NBRC 105215T) is proposed. © 2013 IUMS.