Vogesella alkaliphila sp. nov., isolated from an alkaline soil, and emended description of the genus Vogesella

Abstract

Strain JC141T was isolated from an alkaline soil (pH 8.8) at Mau, Uttar Pradesh, India. Colonies were blue with a metallic sheen; cells stained Gram-negative, and were oxidase- and catalasepositive, but chitinase-negative. Major fatty acids were C16: 1ω7c/C16: 1ω6c, C16: 0 and C18: 0 with minor amounts of C10: 0, C10: 0 3-OH, C12: 0, C14: 0 and C18: 1ω7c. Strain JC141T contained phosphatidylethanolamine and phosphatidylglycerol as major polar lipids, with minor amounts of diphosphatidylglycerol, unidentified aminolipids (AL1-3) and unidentified lipids (L1-4). A bacterial hopane derivative (BHD1) was the major hopanoid. Genomic DNA G+C content was 62.5 mol%. 16S rRNA gene sequence comparisons indicated that strain JC141T represents a member of the genus Vogesella within the family Neisseriaceae of the class Betaproteobacteria. Strain JC141T had a 16S rRNA gene sequence similarity of 98% with Vogesella indigofera ATCC 19706T and < 96.5% with other members of the genus Vogesella. However, strain JC141T showed 45.8% relatedness (based on DNA-DNA hybridization) with V. indigofera LMG 6867T (5ATCC 19706T). Distinct morphological, physiological and genotypic differences from previously described taxa support the classification of strain JC141T as a representative of a novel species in the genus Vogesella, for which the name Vogesella alkaliphila sp. nov. is proposed. The type strain is JC141T (=KCTC 32041T=LMG 27066T). An emended description of the genus Vogesella is also proposed. © G 2013 IUMS.