Genome-wide identification and expression analysis of Arabidopsis GRAM-domain containing gene family in response to abiotic stresses and PGPR treatment

Abstract

Characterization of stress-responsive genes is important to understand the genomics perspective of stress tolerance. In this purview, several gene-families are being identified and characterized in the model and non-model plant species, which has greatly enhanced the knowledge of molecular intricacies associated with stress tolerance. One such gene family is the GRAM-domain containing which have been found to be upregulated in response to plant growth-promoting rhizobacteria (PGPR) treatment followed by salinity stress. Thus, we aimed at understanding the involvement of GRAM domain-containing proteins in abiotic stress response under the influence of rhizobacteria in Arabidopsis thaliana. The study identified fourteen AtGRAM genes in A. thaliana. Further, comprehensive analyses of domain family including phylogenetic studies, domain architecture, gene structure and genomic composition analysis, promoter analysis, homology modelling, and duplication and divergence rates estimation was performed. RNA-Seq derived expression profiling of AtGRAM genes using GENVESTIGATOR in different stresses, developmental stages and hormonal treatments was performed, followed by qRT-PCR analysis under abiotic stresses in response to PGPR. Altogether, the study provided insights into the structure, organization, and evolutionary properties of AtGRAM gene family. Modulation in expression pattern in response to stresses influenced by PGPR-treatment suggests its multifaceted role in cross-talk among abiotic stresses and phytohormones. Further functional characterization of the selected candidate genes would enable understanding of the precise roles of GRAM-genes underlying stress tolerance.