Crystallization and preliminary X-ray crystallographic analysis of a galactose-specific lectin from Dolichos lablab
Crystallization and preliminary X-ray crystallographic analysis of a galactose-specific lectin from Dolichos lablab
| dc.contributor.author | Lavanya Latha, V. | |
| dc.contributor.author | Kulkarni, Kiran A. | |
| dc.contributor.author | Nagender Rao, R. | |
| dc.contributor.author | Siva Kumar, N. | |
| dc.contributor.author | Suguna, K. | |
| dc.date.accessioned | 2022-03-27T04:51:45Z | |
| dc.date.available | 2022-03-27T04:51:45Z | |
| dc.date.issued | 2006-02-01 | |
| dc.description.abstract | The galactose-specific lectin from the seeds of Dolichos lablab has been crystallized using the hanging-drop vapour-diffusion technique. The crystals belong to space group P1, with unit-cell parameters a = 73.99, b = 84.13, c = 93.15 Å, α = 89.92, β = 76.01, γ = 76.99°. X-ray diffraction data to a resolution of 3.0 Å have been collected under cryoconditions (100 K) using a MAR imaging-plate detector system mounted on a rotating-anode X-ray generator. Molecular-replacement calculations carried out using the available structures of legume lectins as search models revealed that the galactose-specific lectin from D. lablab forms a tetramer similar to soybean agglutinin; two such tetramers are present in the asymmetric unit. © 2006 International Union of Crystallography All rights reserved. | |
| dc.identifier.citation | Acta Crystallographica Section F: Structural Biology and Crystallization Communications. v.62(2) | |
| dc.identifier.issn | 17443091 | |
| dc.identifier.uri | 10.1107/S1744309106001448 | |
| dc.identifier.uri | http://scripts.iucr.org/cgi-bin/paper?S1744309106001448 | |
| dc.identifier.uri | https://dspace.uohyd.ac.in/handle/1/7245 | |
| dc.title | Crystallization and preliminary X-ray crystallographic analysis of a galactose-specific lectin from Dolichos lablab | |
| dc.type | Journal. Article | |
| dspace.entity.type |
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