Activation of CDC48 and acetyltransferase encoding genes contributes to enhanced abiotic stress tolerance and improved productivity traits in rice

Abstract

World-wide crop productivity is highly impacted by various extreme environmental conditions. In the present investigation, activation tagged (AT) line A10-Ds-RFP6 of rice endowed with improved agronomic attributes was tested for its tolerance ability against drought and salinity stress conditions as well as identification of genes associated with these traits. Under both drought and salinity stress conditions, A10-Ds-RFP6 line exhibited increased seed germination rates and improved plant growth characteristics at seedling, vegetative and reproductive stages as compared to wild-type (WT) plants. Moreover, A10-Ds-RFP6 revealed effective antioxidant systems resulting in decreased accumulation of reactive oxygen species and delayed stress symptoms compared to WT plants. Reduced accumulation of malondialdehyde with concomitant increase in proline and soluble sugars in A10-Ds-RFP6 line further endorse its improved stress tolerance levels. Furthermore, A10-Ds-RFP6 disclosed enhanced plant water content, photosynthetic efficiency, stomatal conductance, water use efficiency and maximum quantum yield compared to WT plants. TAIL and qRT-PCR analyses of AT rice line revealed the integration site of Ds element in the genome and increased expression levels of CDC48 and acetyltransferase genes involved in various aspects of plant development and stress tolerance. As such, the promising AT line plausibly serve as a rare genetic resource for fortifying stress tolerance and productivity traits of elite rice cultivars.