Effects of melatonin, p-chlorophenylalanine, and α-methylparatyrosine on plasma gonadotropin level and ovarian activity in the catfish, Heteropneustes fossilis: A study correlating changes in hypothalamic monoamines

Abstract

The effects of (ip, 10 injections over 20 days) of melatonin (75 μg 100 g-1 BW), the serotonin (5-HT)-synthesis blocker, para-cholorophenylalanine (p-CPA, 10 mg 100g-1 BW) and the catecholamine-synthesis blocker, α-methylparatyrosine (α-MPT, 10 mg 100 g-1 BW) on gonadotropin (GTH) secretion and ovarian activity were studied in Heteropneustes fossilis during late preparatory to early prespawning (April-May). The treatments resulted in significant reductions of plasma GTH and estradiol-17β levels, the gonadosomatic index, frequency distribution of vitellogenic and postvitellogenic oocytes, and ovarian and serum 32p-labelled alkali-labile phosphoprotein (a marker of vitellogenic activity). Most of the oocytes were nonvitellogenic or had undergone atretic changes. The hepatic 32-phosphoprotein content increased significantly over the saline control value. The effects were similar and pronounced in the p-CPA and melatonin-treated groups but were moderate in the α-MPT-treated group. Hypothalamic 5-HT content and turnover were significantly inhibited in the p-CPA and melatonin-treated groups but the content and turnover of catecholamines were not. The α-MPT treatment decreased significantly the content and turnover of dopamine (DA), noradrenaline (NA), and adrenaline (A) but did not influence the 5-HT content or turnover. These results suggest that 5-HT, NA and A are stimulatory to GTH secretion and that melatonin may act on the serotonergic system to inhibit the pituitary-gonadal axis. © 1995 Kugler Publications.