A low-cost high-throughput method for plant genomic DNA isolation

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dc.contributor.author Gupta, Prateek
dc.contributor.author Salava, Hymavathi
dc.contributor.author Sreelakshmi, Yellamaraju
dc.contributor.author Sharma, Rameshwar
dc.date.accessioned 2022-03-27T03:48:59Z
dc.date.available 2022-03-27T03:48:59Z
dc.date.issued 2020-01-01
dc.description.abstract Many of the functional genomics methods require isolation of genomic DNA from large population of plants. The selection of DNA isolation protocols depends on several factors such as choice of starting material, ease of handling, time and labor required for isolation, the final quantity as well as the quality of genomic DNA. We outline here a high-throughput method of DNA extraction from different plant species including cereal crops. The protocol can be used for extraction of DNA in single tubes as well as for large formats in 96-well plates. The protocol includes steps for eliminating interfering secondary products such as phenolics. This protocol can be applied for high-throughput isolation of DNA for varied applications such as TILLING, mapping, fingerprinting, etc. as a cost-effective protocol compared to commercial kits.
dc.identifier.citation Methods in Molecular Biology. v.2072
dc.identifier.issn 10643745
dc.identifier.uri 10.1007/978-1-4939-9865-4_1
dc.identifier.uri http://link.springer.com/10.1007/978-1-4939-9865-4_1
dc.identifier.uri https://dspace.uohyd.ac.in/handle/1/5611
dc.subject Cereals
dc.subject Corn
dc.subject DNA isolation
dc.subject Genomic DNA
dc.subject Maize
dc.title A low-cost high-throughput method for plant genomic DNA isolation
dc.type Book Series. Book Chapter
dspace.entity.type
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