In vitro evaluation of niobia added soda lime borosilicate bioactive glasses

Abstract

In the present study, systematic and detailed investigations have been reported, in terms of bioactivity, cytocompatibility and mechanical hardness of a synthetic material that is proposed as a bone implant material in in-vitro conditions. The objective of the present study is to explore the effect of niobium addition on bioactivity and cytocompatibility of soda lime borosilicate bioglasses. Synthesis of bioglasses is carried out by conventional melt-quenching method. Simulated body fluid is prepared by Tas method. The bioactivity study was done for twenty-one days of immersion in simulated body fluid. XRD, FTIR, and SEM-EDS are showed formation of hydroxyapatite layer on the niobium added soda lime borosilicate glass even at a higher concentration (10 mol %) of niobium content. Degradation test was performed as per ISO 10993. The results obtained from degradation studies have demonstrated that weight loss of niobia mixed soda lime borosilicate glasses decreased with the increase of niobia. Cytocompatibility study has been studied by MTT Assay with osteoblast cells (MG-63). Cell viability is not affected by the addition of niobium content. Niobium added soda lime borosilicate glasses are both bioactive and cytocompatible. Hence it can be concluded that niobia mixed borosilicate glasses may be used as potential material in bone tissue engineering.