Rhodoplanes oryzae sp. nov., a phototrophic alphaproteobacterium isolated from the rhizosphere soil of paddy

Abstract

A Gram-stain-negative, rod-shaped, phototrophic bacterium, strain JA793T, was isolated from rhizosphere soil of paddy. The strain was capable of growing phototrophically and chemotrophically. Bacteriochlorophyll-a and carotenoids of the spirilloxanthin series were present as photosynthetic pigments. The major fatty acid of strain JA793T was C18: 1ω7c/C18: 1ω6c ( > 65.7 %), with minor amounts of C16: 0, C16: 1ω7c/C16: 1ω6c, C20: 2ω6,9c, C16: 0 3-OH, C14: 0 and C18: 0 also present. Ubiquinone-10 and rhodoquinone-10 were present as primary quinones. Phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine were the major polar lipids, while minor amounts of amino lipids and unidentified lipids were also present. The G+C content of genomic DNA of strain JA793T was 68.7 mol%. 16S rRNA gene-based EzTaxon-e BLAST search analysis of strain JA793Tindicated highest sequence similarity with members of the genus Rhodoplanes in the family Hyphomicrobiaceae of the class Alphaproteobacteria. Strain JA793T had high sequence similarity with Rhodoplanes elegans AS130T (98.6 %), Rhodoplanes roseus 941T (98 %), Rhodoplanes pokkaliisoli JA415T (97.5 %) and Rhodoplanes piscinae JA266T (97.3 %) and other members of the genus Rhodoplanes ( < 97 %). However, strain JA266T was related by < 59 % (based on DNA-DNA hybridization) to Rhodoplanes elegans DSM 11907T (= AS130T), Rhodoplanes roseus DSM 5909T (= 941T), Rhodoplanes pokkaliisoli JA415Tand Rhodoplanes piscinae JA266T. The genomic information was well supported by phenotypic and chemotaxonomic data to classify strain JA793Tas a representative of a novel species in the genus Rhodoplanes, for which the name Rhodoplanes oryzae sp. nov. is proposed. The type strain is JA793T (= NBRC 109406T = KCTC 15260T). © 2014 IUMS.