Rhodovulum algae sp. nov., isolated from an algal mat

Abstract

A reddish-brown-pigmented, phototrophic bacterium, designated strain JA877T, was isolated from a brown algae mat sample collected from Jalandhar beach, Gujarat, India. On the basis of the 16S rRNA gene sequence, strain JA877T belongs to the class Alphaproteobacteria and is closely related to the type strains Rhodovulum viride JA756T(99.0 %), Rhodovulum sulfidophilum Hansen W4T(98.9 %), Rhodovulum visakhapatnamense JA181T(98.8 %), Rhodovulum kholense JA297T(97.5 %) and Rhodovulum salis JA746T(97.0). However, strain JA877T showed only 20–45% relatedness with its phylogenetic neighbours and had a ΔTmbetween 5.8 and 7.0 °C. The major respiratory quinone was ubiquinone-10 (Q10), and the polar lipid profile was composed of the major components phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid, two unidentified sulfolipids and five unidentified lipids. The major fatty acids were C18: 1ω5c, C18: 1ω7c/C18: 1ω6c, C16: 0 and C18: 0. The DNA G+C content was 64.5 mol%. On the basis of 16S rRNA gene sequence analysis, physiological data, and chemotaxonomic and molecular differences, strain JA877T is significantly different from other species of the genus Rhodovulum and represents a novel species, for which the name Rhodovulum algae sp. nov. is proposed. The type strain is JA877T(=LMG 29228T= KCTC 42963T).