Catabolism of l-phenylalanine and l-tyrosine by Rhodobacter sphaeroides OU5 occurs through 3,4-dihydroxyphenylalanine
Catabolism of l-phenylalanine and l-tyrosine by Rhodobacter sphaeroides OU5 occurs through 3,4-dihydroxyphenylalanine
dc.contributor.author | Ranjith, N. K. | |
dc.contributor.author | Sasikala, Ch | |
dc.contributor.author | Ramana, Ch V. | |
dc.date.accessioned | 2022-03-27T03:46:05Z | |
dc.date.available | 2022-03-27T03:46:05Z | |
dc.date.issued | 2007-07-01 | |
dc.description.abstract | Rhodobacter sphaeroides OU5 utilized l-phenylalanine as sole source of nitrogen for growth. The metabolites of l-phenylalanine catabolism, i.e. 4-hydroxy phenylalanine (l-tyrosine), 3,4-dihydroxyphenylalanine (DOPA), 3,4-dihydroxyphenyl-pyruvic acid (DOPP), 3,4-dihydroxyphenyllactic acid (DOPLA), 3,4-dihydroxyphenyl-acetic acid (DOPAc) and 3,4-dihydroxybenzoic acid (PC), were identified using liquid chromatography-mass spectroscopy (LC-MS). With 2-oxoglutarate as an amino acceptor, DOPA aminotransferase activity was observed with cell-free extracts and the product DOPP was confirmed through mass analysis. Reductive deamination of DOPA also occurred in the absence of 2-oxoglutarate, whose products were 3,4-dihydroxyphenylpropionic acid (DPPA) and ammonia. The enzyme DOPA-reductive deaminase (DOPARDA) was purified to its homogeneity and characterized. DOPARDA has an obligate requirement for NADH and is functional at low concentrations of the substrate ( < 150 μM). The molecular mass of the purified enzyme was ≈274 kD and the enzyme could be a heterotetramer of 110, 82, 43 and 39 kD subunits as determined by SDS-PAGE. © 2007 Elsevier Masson SAS. All rights reserved. | |
dc.identifier.citation | Research in Microbiology. v.158(6) | |
dc.identifier.issn | 09232508 | |
dc.identifier.uri | 10.1016/j.resmic.2007.04.008 | |
dc.identifier.uri | https://www.sciencedirect.com/science/article/abs/pii/S0923250807001131 | |
dc.identifier.uri | https://dspace.uohyd.ac.in/handle/1/5324 | |
dc.subject | 3-4 Dihydroxy phenylalanine (DOPA) | |
dc.subject | Aromatic hydrocarbons | |
dc.subject | Biodegradation | |
dc.subject | DOPA aminotransferase (DOPAATS) | |
dc.subject | DOPA reductive deaminase (DOPARDA) | |
dc.subject | l-phenylalanine | |
dc.subject | Rhodobacter sphaeroides OU5 | |
dc.title | Catabolism of l-phenylalanine and l-tyrosine by Rhodobacter sphaeroides OU5 occurs through 3,4-dihydroxyphenylalanine | |
dc.type | Journal. Article | |
dspace.entity.type |
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