An adaptable live-cell imaging protocol to analyze organelle morphology in Saccharomyces cerevisiae

dc.contributor.author	Deolal, Pallavi
dc.contributor.author	Mishra, Krishnaveni
dc.date.accessioned	2022-03-27T04:51:27Z
dc.date.available	2022-03-27T04:51:27Z
dc.date.issued	2022-03-18
dc.description.abstract	The protocol describes semiautomated live cell imaging in budding yeast. A key feature of the protocol is immobilizing cells in a culture dish, which allows for longer imaging times, changing culture media, or drug treatments. We describe steps for image acquisition and deconvolution, followed by manual analysis of quantifiable parameters to represent morphological changes in nuclear shape. We compare wild type with ssf1Δ, which is known to alter nuclear morphology. The protocol can be adapted to other organelles and processes. For complete details on the use and execution of this profile, please refer to Male et al., 2020, Deolal et al. (2021).
dc.identifier.citation	STAR Protocols. v.3(1)
dc.identifier.uri	10.1016/j.xpro.2022.101124
dc.identifier.uri	https://www.sciencedirect.com/science/article/abs/pii/S2666166722000041
dc.identifier.uri	https://dspace.uohyd.ac.in/handle/1/7147
dc.subject	Cell Biology
dc.subject	Genetics
dc.subject	Microbiology
dc.subject	Microscopy
dc.subject	Model Organisms
dc.title	An adaptable live-cell imaging protocol to analyze organelle morphology in Saccharomyces cerevisiae
dc.type	Journal. Article
dspace.entity.type

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