A novel meta-cleavage product hydrolase from Flavobacterium sp. ATCC27551

dc.contributor.author Khajamohiddin, Syed
dc.contributor.author Babu, Pakala Suresh
dc.contributor.author Chakka, Deviprasanna
dc.contributor.author Merrick, Mike
dc.contributor.author Bhaduri, Anirban
dc.contributor.author Sowdhamini, Ramanathan
dc.contributor.author Siddavattam, Dayananda
dc.date.accessioned 2022-03-27T00:57:47Z
dc.date.available 2022-03-27T00:57:47Z
dc.date.issued 2006-12-22
dc.description.abstract The organophosphate degrading (opd) gene cluster of plasmid pPDL2 of Flavobacterium sp. ATCC27551 contains a novel open-reading frame, orf243. This was predicted to encode an α/β hydrolase distantly related to the meta-fission product (MFP) hydrolases such as XylF, PhnD, and CumD. By homology modeling Orf243 has most of the structural features of MFP hydrolases including the characteristic active site catalytic triad. The purified protein (designated MfhA) is a homotetramer and shows similar affinity for 2-hydroxy-6-oxohepta-2,4-dienoate (HOHD), 2-hydroxymuconic semialdehyde (HMSA), and 2-hydroxy-5-methylmuconic semialdehyde (HMMSA), the meta-fission products of 3-methyl catechol, catechol, and 4-methyl catechol. The unique catalytic properties of MfhA and the presence near its structural gene of cis-elements required for transposition suggest that mfhA has evolved towards encoding a common hydrolase that can act on meta-fission products containing either aldehyde or ketone groups. © 2006 Elsevier Inc. All rights reserved.
dc.identifier.citation Biochemical and Biophysical Research Communications. v.351(3)
dc.identifier.issn 0006291X
dc.identifier.uri 10.1016/j.bbrc.2006.10.080
dc.identifier.uri https://www.sciencedirect.com/science/article/abs/pii/S0006291X06023357
dc.identifier.uri https://dspace.uohyd.ac.in/handle/1/3491
dc.subject α/β Hydrolase
dc.subject Flavobacterium sp. ATCC27551
dc.subject Hydrolase
dc.subject meta-Fission product
dc.subject meta-Pathway
dc.title A novel meta-cleavage product hydrolase from Flavobacterium sp. ATCC27551
dc.type Journal. Article
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