Hymenobacter roseus sp. nov., isolated from sand

Abstract

Strain JC245T was isolated from a sand sample, and appeared as dark pink colonies on agar plates with cells staining Gram-negative. Catalase and oxidase activities were positive. Casein was hydrolysed while chitin, gelatin and starch were not. Major ( > 5 %) fatty acids were iso-C15: 0, iso-C15: 0 3-OH, C16: 1ω5c, C16: 1ω6c/C16: 1ω7c, anteiso-C17: 1 B/iso-C17: 1 I and iso-C17: 03-OH. Strain JC245T contained phosphatidylethanolamine and two unidentified lipids as the major polar lipids, with minor amounts of four unidentified lipids and an unidentified amino lipid. Bacterial hopane derivatives and adenosylhopane were the major hopanoids. Hydroxyflexixanthin was identified as one of the major carotenoids of strain JC245T along with five unidentified carotenoids. The genomic DNA G+C content was 52.5 mol%. 16S rRNA gene sequence comparisons indicated that strain JC245T represents a member of the genus Hymenobacter within the family Cytophagaceae of the phylum Bacteroidetes. Strain JC245T shared the highest 16S rRNA gene sequence similarity with Hymenobacter roseosalivariusAA-718T (98.3 %) and other members of the genus Hymenobacter ( < 95.1 %). However, strain JC245T showed 21±2 % relatedness (based on DNA–DNA hybridization) with H. roseosalivarius DSM 11622T. Distinct morphological, physiological and genotypic differences from previously described taxa support the classification of strain JC245T as a representative of a novel species in the genus Hymenobacter, for which the name Hymenobacter roseussp. nov. is proposed. The type strain is JC245T (= KCTC 42090T = LMG 28260T).