Anti-leukemic effects of gallic acid on human leukemia K562 cells: Downregulation of COX-2, inhibition of BCR/ABL kinase and NF-κB inactivation

dc.contributor.author Chandramohan Reddy, T.
dc.contributor.author Bharat Reddy, D.
dc.contributor.author Aparna, A.
dc.contributor.author Arunasree, Kalle M.
dc.contributor.author Gupta, Geetika
dc.contributor.author Achari, Chandrani
dc.contributor.author Reddy, G. V.
dc.contributor.author Lakshmipathi, V.
dc.contributor.author Subramanyam, A.
dc.contributor.author Reddanna, P.
dc.date.accessioned 2022-03-27T01:02:15Z
dc.date.available 2022-03-27T01:02:15Z
dc.date.issued 2012-04-01
dc.description.abstract Gallic acid (GA) induces apoptosis in various cancer cell lines. In this study, we investigated the apoptotic activity induced by GA on chronic myeloid leukemia (CML) cell line-K562 and the underlying mechanism. GA reduced the viability of K562 cells in a dose and time dependent manner. GA led to G 0/G 1 phase arrest in K562 cells by promoting p21 and p27 and inhibiting the levels of cyclin D and cyclin E. Further studies indicated apoptosis with impaired mitochondrial function as a result of deranged Bcl-2/Bax ratio, leakage of cytochrome c and PARP cleavage along with DNA fragmentation and by up-regulating the expression of caspase-3. GA also activated the protein expressions of fatty acid synthase ligand and caspase-8. GA is more effective in imatinib resistant-K562 (IR-K562) cells (IC 50 4μM) than on K562 cells (IC 50 33μM). GA inhibited cyclooxygenase-2 (COX-2) in K562 as well as IR-K562 cells appears to be COX-2 involved in the suppression of growth. Interestingly, GA also inhibited BCR/ABL tyrosine kinase and NF-κB. In conclusion, GA induced apoptosis in K562 cells involves death receptor and mitochondrial-mediated pathways by inhibiting BCR/ABL kinase, NF-κB activity and COX-2. © 2012 Elsevier Ltd.
dc.identifier.citation Toxicology in Vitro. v.26(3)
dc.identifier.issn 08872333
dc.identifier.uri 10.1016/j.tiv.2011.12.018
dc.identifier.uri https://www.sciencedirect.com/science/article/abs/pii/S0887233311003444
dc.identifier.uri https://dspace.uohyd.ac.in/handle/1/3942
dc.subject BCR/ABL kinase
dc.subject Chronic myeloid leukemia (K562) cell line
dc.subject Cyclooxygenase-2
dc.subject Gallic acid
dc.subject Imatinib resistant-K562 cells
dc.subject Nf-κB
dc.title Anti-leukemic effects of gallic acid on human leukemia K562 cells: Downregulation of COX-2, inhibition of BCR/ABL kinase and NF-κB inactivation
dc.type Journal. Article
dspace.entity.type
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