A novel transcriptional regulator, Sll1130, negatively regulates heat-responsive genes in Synechocystis sp. PCC6803
A novel transcriptional regulator, Sll1130, negatively regulates heat-responsive genes in Synechocystis sp. PCC6803
dc.contributor.author | Krishna, Pilla Sankara | |
dc.contributor.author | Rani, Balaga Radha | |
dc.contributor.author | Mohan, M. Karthik | |
dc.contributor.author | Suzuki, Iwane | |
dc.contributor.author | Shivaji, Sisinthy | |
dc.contributor.author | Prakash, Jogadhenu S.S. | |
dc.date.accessioned | 2022-03-27T05:20:19Z | |
dc.date.available | 2022-03-27T05:20:19Z | |
dc.date.issued | 2013-02-01 | |
dc.description.abstract | A conserved hypothetical protein, Sll1130, is a novel transcription factor that regulates the expression of major heat-responsive genes in Synechocystis sp. PCC6803. Synechocystis exhibited an increased thermotolerance due to disruption of sll1130. Δsll1130 cells recovered much faster than wild-type cells after they were subjected to heat shock (50°C) for 30 min followed by recovery at 34°C for 48 h. In Δsll1130 cultures, 70% of the cells were viable compared with the wild-type culture in which only 30% of the cells were viable. DNA microarray analysis revealed that in Δsll1130, expression of the heat-responsive genes such as htpG, hspA, isiA, isiB and several hypothetical genes were up-regulated. Sll1130 binds to a conserved inverted-repeat (GGCGATCGCC) located in the upstream region of the above genes. In addition, both the transcript and protein levels of sll1130 were immediately down-regulated upon shift of wild-type cells from 34 to 42°C. Collectively the results of the present study suggest that Sll1130 is a heat-responsive transcriptional regulator that represses the expression of certain heat-inducible genes at optimum growth temperatures. Upon heat shock, a quick drop in the Sll1130 levels leads to de-repression of the heat-shock genes and subsequent thermal acclimation. On the basis of the findings of the present study, we present a model which describes the heat-shock response involving Sll1130. © The Authors Journal compilation © 2013 Biochemical Society. | |
dc.identifier.citation | Biochemical Journal. v.449(3) | |
dc.identifier.issn | 02646021 | |
dc.identifier.uri | 10.1042/BJ20120928 | |
dc.identifier.uri | https://portlandpress.com/biochemj/article/449/3/751/46142/A-novel-transcriptional-regulator-Sll1130 | |
dc.identifier.uri | https://dspace.uohyd.ac.in/handle/1/8142 | |
dc.subject | Cis-regulatory element | |
dc.subject | DNA microarray | |
dc.subject | Heat acclimation | |
dc.subject | Sll1130 | |
dc.subject | Transcription factor | |
dc.title | A novel transcriptional regulator, Sll1130, negatively regulates heat-responsive genes in Synechocystis sp. PCC6803 | |
dc.type | Journal. Article | |
dspace.entity.type |
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