A novel transcriptional regulator, Sll1130, negatively regulates heat-responsive genes in Synechocystis sp. PCC6803

dc.contributor.author Krishna, Pilla Sankara
dc.contributor.author Rani, Balaga Radha
dc.contributor.author Mohan, M. Karthik
dc.contributor.author Suzuki, Iwane
dc.contributor.author Shivaji, Sisinthy
dc.contributor.author Prakash, Jogadhenu S.S.
dc.date.accessioned 2022-03-27T05:20:19Z
dc.date.available 2022-03-27T05:20:19Z
dc.date.issued 2013-02-01
dc.description.abstract A conserved hypothetical protein, Sll1130, is a novel transcription factor that regulates the expression of major heat-responsive genes in Synechocystis sp. PCC6803. Synechocystis exhibited an increased thermotolerance due to disruption of sll1130. Δsll1130 cells recovered much faster than wild-type cells after they were subjected to heat shock (50°C) for 30 min followed by recovery at 34°C for 48 h. In Δsll1130 cultures, 70% of the cells were viable compared with the wild-type culture in which only 30% of the cells were viable. DNA microarray analysis revealed that in Δsll1130, expression of the heat-responsive genes such as htpG, hspA, isiA, isiB and several hypothetical genes were up-regulated. Sll1130 binds to a conserved inverted-repeat (GGCGATCGCC) located in the upstream region of the above genes. In addition, both the transcript and protein levels of sll1130 were immediately down-regulated upon shift of wild-type cells from 34 to 42°C. Collectively the results of the present study suggest that Sll1130 is a heat-responsive transcriptional regulator that represses the expression of certain heat-inducible genes at optimum growth temperatures. Upon heat shock, a quick drop in the Sll1130 levels leads to de-repression of the heat-shock genes and subsequent thermal acclimation. On the basis of the findings of the present study, we present a model which describes the heat-shock response involving Sll1130. © The Authors Journal compilation © 2013 Biochemical Society.
dc.identifier.citation Biochemical Journal. v.449(3)
dc.identifier.issn 02646021
dc.identifier.uri 10.1042/BJ20120928
dc.identifier.uri https://portlandpress.com/biochemj/article/449/3/751/46142/A-novel-transcriptional-regulator-Sll1130
dc.identifier.uri https://dspace.uohyd.ac.in/handle/1/8142
dc.subject Cis-regulatory element
dc.subject DNA microarray
dc.subject Heat acclimation
dc.subject Sll1130
dc.subject Transcription factor
dc.title A novel transcriptional regulator, Sll1130, negatively regulates heat-responsive genes in Synechocystis sp. PCC6803
dc.type Journal. Article
dspace.entity.type
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