Description of Jeotgalibacillus alkaliphilus sp. nov., isolated from a solar salt pan, and Jeotgalibacillus terrae sp. nov., a name to replace ‘Jeotgalibacillus soli’ Chen et al. 2010

Abstract

A Gram-stain-positive, non-motile, rod-shaped bacterium (strain JC303T) isolated from a salt pan was identified based on 16S rRNA gene sequence analysis as a member of the genus Jeotgalibacillus. It was related most closely to Jeotgalibacillus salarius ASL-1T (99.1% similarity), Jeotgalibacillus alimentarius YKJ-13T (97.9%), Jeotgalibacillus soli JSM 081008 (97.9%), Jeotgalibacillus malaysiensis D5T (97.8%), Jeotgalibacillus marinus DSM 1297T (96.3%), Jeotgalibacillus campisalis SF-57T (96.1%) and J. soli P9T (94.9%). Genomic relatedness based on DNA–DNA hybridization of strain JC303T with the type strains of the closest related species was less than 40%. Diphosphatidylglycerol, three aminophospholipids, an unidentified aminoglycolipid, two unidentified phospholipids and an unidentified lipid were the polar lipids of strain JC303T. Major ( > 10%) fatty acids were anteiso-C15: 0, iso-C15: 0 and iso-C14: 0. Cell-wall amino acids contained peptidoglycan with L-lysine as the diagnostic diamino acid. Strain JC303T contained MK-7 as the predominant (96%) menaquinone with the presence of a significant amount (4%) of MK-8. The DNA G+C content was 43 mol%. On the basis of morphological, physiological, genotypic, phylogenetic and chemotaxonomic analyses, strain JC303T is considered to represent a novel species of the genus Jeotgalibacillus, for which the name Jeotgalibacillus alkaliphilus sp. nov. is proposed. The type strain is JC303T (=KCTC 33662T=LMG 28756T). In addition, we propose to rename J. soli (Chen et al., 2010), an illegitimate homonym of the validly published name Jeotgalibacillus soli (Cunha et al., 2012) as Jeotgalibacillus terrae sp. nov. with type strain JSM 081008T (=DSM 22174T=KCTC 13528T).