Purification and characterization of UV-B induced phenylalanine ammonia- lyase from rice seedlings

dc.contributor.author	Sarma, Annamraju D.
dc.contributor.author	Sharma, Rameshwar
dc.date.accessioned	2022-03-27T03:49:34Z
dc.date.available	2022-03-27T03:49:34Z
dc.date.issued	1999-03-10
dc.description.abstract	On exposure of rice seedlings to UV-B radiations, a four fold induction of PAL activity was observed. Purification of induced PAL revealed the presence of a single isoform with a molecular mass 320 kD and a subunit Mr of 84 kD. The enzyme exhibited Michaelis-Menten kinetics with a Km of 500 μM, optimal pH 8.9 and an activation energy 1.9 kcal. The induced enzyme was sensitive to inhibition by phenylpropanoid intermediates involved in flavonol and anthocyanin biosynthetic pathway.
dc.identifier.citation	Phytochemistry. v.50(5)
dc.identifier.issn	00319422
dc.identifier.uri	10.1016/S0031-9422(98)00608-6
dc.identifier.uri	https://www.sciencedirect.com/science/article/abs/pii/S0031942298006086
dc.identifier.uri	https://dspace.uohyd.ac.in/handle/1/5653
dc.subject	Gramineae
dc.subject	Oryza sativa
dc.subject	Phenylalanine ammonia-lyase
dc.subject	Rice
dc.subject	UV-B radiation
dc.title	Purification and characterization of UV-B induced phenylalanine ammonia- lyase from rice seedlings
dc.type	Journal. Article
dspace.entity.type

Now showing 1 - 1 of 1