Site specific phosphorylation of cytochrome c oxidase subunits I, IVi1 and Vb in rabbit hearts subjected to ischemia/reperfusion
Site specific phosphorylation of cytochrome c oxidase subunits I, IVi1 and Vb in rabbit hearts subjected to ischemia/reperfusion
| dc.contributor.author | Fang, Ji Kang | |
| dc.contributor.author | Prabu, Subbuswamy K. | |
| dc.contributor.author | Sepuri, Naresh B. | |
| dc.contributor.author | Raza, Haider | |
| dc.contributor.author | Anandatheerthavarada, Hindupur K. | |
| dc.contributor.author | Galati, Domenico | |
| dc.contributor.author | Spear, Joseph | |
| dc.contributor.author | Avadhani, Narayan G. | |
| dc.date.accessioned | 2022-03-27T04:52:11Z | |
| dc.date.available | 2022-03-27T04:52:11Z | |
| dc.date.issued | 2007-04-03 | |
| dc.description.abstract | We have mapped the sites of ischemia/reperfusion-induced phosphorylation of cytochrome c oxidase (CcO) subunits in rabbit hearts by using a combination of Blue Native gel/Tricine gel electrophoresis and nano-LC-MS/MS approaches. We used precursor ion scanning combined with neutral loss scanning and found that mature CcO subunit I was phosphorylated at tandem Ser115/Ser116 positions, subunit IVi1 at Thr52 and subunit Vb at Ser40. These sites are highly conserved in mammalian species. Molecular modeling suggests that phosphorylation sites of subunit I face the inter membrane space while those of subunits IVi1 and Vb face the matrix side. © 2007 Federation of European Biochemical Societies. | |
| dc.identifier.citation | FEBS Letters. v.581(7) | |
| dc.identifier.issn | 00145793 | |
| dc.identifier.uri | 10.1016/j.febslet.2007.02.042 | |
| dc.identifier.uri | http://doi.wiley.com/10.1016/j.febslet.2007.02.042 | |
| dc.identifier.uri | https://dspace.uohyd.ac.in/handle/1/7340 | |
| dc.subject | Cytochrome c oxidase | |
| dc.subject | Myocardial ischemia/reperfusion | |
| dc.subject | Nano-LC-MS/MS analysis | |
| dc.subject | Protein kinase A | |
| dc.subject | Subunit phosphorylation | |
| dc.title | Site specific phosphorylation of cytochrome c oxidase subunits I, IVi1 and Vb in rabbit hearts subjected to ischemia/reperfusion | |
| dc.type | Journal. Article | |
| dspace.entity.type |
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