Synthesis and biological evaluation of photoaffinity labeled fusidic acid analogues

Abstract

Novel photoaffinity labeled fusidic acid analogues were obtained by a synthetic sequence employing a Wittig reaction between a fusidic acid aldehyde and benzyl bromides in the key step. Three commonly used photoreactive groups, benzophenone, trifluoromethyldiazirine, and aryl azide, were used. The photoaffinity labeled fusidic acid analogues demonstrated a potent antibacterial activity (MIC 0.016-4 μg/mL) and therefore represent a potential tool for the elucidation of the interactions between fusidic acid and its receptor EF-G. © 2006 American Chemical Society.