Measurement of mitochondrial respiration in isolated protoplasts: Cytochrome and alternative pathways

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dc.contributor.author Sunil, Bobba
dc.contributor.author Raghavendra, Agepati S.
dc.date.accessioned 2022-03-27T03:50:23Z
dc.date.available 2022-03-27T03:50:23Z
dc.date.issued 2017-01-01
dc.description.abstract The electron partitioning between COX and AOX pathways of mitochondria and their coordination is necessary to meet the energy demands as well as to maintain optimized redox status in plants under varying environmental conditions. The relative contribution of these two pathways to total respiration is an important measure during a given stress condition. We describe in detail the procedure that allows the measurement of the parameters of COX and AOX pathway of respiration in mesophyll protoplasts using Clark-type O2 electrode. This chapter also lists the steps for rapid isolation procedure for mesophyll protoplasts from pea leaves. The advantages and limitations of the use of metabolic inhibitors and the protoplasts for measuring the respiration are also briefly discussed.
dc.identifier.citation Methods in Molecular Biology. v.1670
dc.identifier.issn 10643745
dc.identifier.uri 10.1007/978-1-4939-7292-0_22
dc.identifier.uri http://link.springer.com/10.1007/978-1-4939-7292-0_22
dc.identifier.uri https://dspace.uohyd.ac.in/handle/1/5712
dc.subject Alternative oxidase (AOX) pathway
dc.subject Alternative pathway engagement
dc.subject Clark-type O electrode 2
dc.subject Cytochrome oxidase (COX) pathway
dc.subject Mesophyll cell protoplasts
dc.subject Mitochondrial electron transport
dc.subject Mitochondrial inhibitors
dc.subject Pisum sativum
dc.title Measurement of mitochondrial respiration in isolated protoplasts: Cytochrome and alternative pathways
dc.type Book Series. Book Chapter
dspace.entity.type
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