An immuno-affinity method for the purification of mannose 6-phosphate receptor proteins

dc.contributor.author	Suresh, Koduru
dc.contributor.author	Nadimpalli, Siva Kumar
dc.date.accessioned	2022-03-27T04:51:46Z
dc.date.available	2022-03-27T04:51:46Z
dc.date.issued	2003-09-30
dc.description.abstract	In a recent study, we have developed an ELISA method to quantify the mannose 6-phosphate receptor (MPR) proteins [J. Biochem. Biophys. Methods 52 (2002) 111]. In the present study, we have used the goat MPR 300 antibody and peptide specific antibodies to human MPR 46 to develop simple and efficient immuno-affinity matrices, which can be used to purify the MPR proteins from goat liver in a single step. The identity of the immuno-affinity purified receptors is confirmed by their molecular masses as well as by their immunoreactivity. © 2003 Elsevier B.V. All rights reserved.
dc.identifier.citation	Journal of Biochemical and Biophysical Methods. v.57(3)
dc.identifier.issn	0165022X
dc.identifier.uri	10.1016/S0165-022X(03)00148-9
dc.identifier.uri	https://www.sciencedirect.com/science/article/abs/pii/S0165022X03001489
dc.identifier.uri	https://dspace.uohyd.ac.in/handle/1/7251
dc.subject	Immuno-affinity
dc.subject	MPR proteins
dc.subject	Peptide-specific antibodies
dc.title	An immuno-affinity method for the purification of mannose 6-phosphate receptor proteins
dc.type	Journal. Article
dspace.entity.type

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