Shewanella chilikensis sp. nov., a moderately alkaliphilic gammaproteobacterium isolated from a lagoon

Abstract

A Gram-negative, motile, rod-shaped, facultatively anaerobic bacterium was isolated from sediment of Chilika Lagoon, a brackish water lagoon in India. The strain, designated JC5T, was able to grow in the presence of 0-8.0 % NaCl and at pH 7.0-10.0. The isolate was positive for oxidase and catalase and exhibited α-haemolysis. The major fatty acids were iso-C15:0 (18.3 %), C16:0 (11.3 %), C17:1ω8c (13.1 %) and a summed feature, C16:1ω7c and/or C16:1ω6c (15.1 %). The genomic DNA G+C content was 54.6 mol%. A phylogenetic tree based on the 16S rRNA gene sequences showed that strain JC5T forms a lineage within the genus Shewanella and is closely related to Shewanella haliotis DW01T (98.0 %), Shewanella algae ATCC 51192T (97.6 %) and Shewanella marina C4T (95.8 %). Further, genomic DNA-DNA hybridization of strain JC5T with S. haliotis DW01T and S. algae ATCC 51192T showed relatedness of only 42 and 23 %, respectively. On the basis of phenotypic and molecular genetic evidence, strain JC5T represents a novel species of the genus Shewanella, for which the name Shewanella chilikensis sp. nov. is proposed. The type strain is JC5T (=CCUG 57101T =NBRC 105217T =KCTC 22540T). © 2009 IUMS.