Expression and immunolocalization of 20β-hydroxysteroid dehydrogenase during testicular cycle and after hCG induction, in vivo in the catfish, Clarias gariepinus

Abstract

The maturation inducing hormone, 17α,20β-dihydroxy-4-pregnen-3-one (17α,20β-DP) is required for the meiotic maturation and is produced from the precursor 17α-hydroxyprogesterone by the enzyme 20β-hydroxysteroid dehydrogenase (20β-HSD) in several teleosts. Central role of 20β-HSD in ovarian cycle and final oocyte maturation is well studied when compared to spermatogenesis. In the present study, we investigated the localization and expression of 20β-HSD in testicular cycle and gonadotropin induced sperm maturation. During testicular ontogeny, 20β-HSD expression was detectable at 50 and 100. days post-hatch (dph), while the expression was high at 150. dph. In testicular cycle, highest levels of mRNA and protein of 20β-HSD were observed during spawning phase. Intraperitoneal injection of human chorionic gonadotropin (hCG) to prespawning catfish elevated both 20β-HSD transcripts and protein levels when compared to saline treated controls in a time-dependent manner. Serum 17α,20β-DP levels, measured during different phases of testicular cycle as well as following the treatment of hCG, showed a positive correlation with the expression of 20β-HSD. Immunolocalization revealed the presence of 20β-HSD protein predominantly in interstitial cells and spermatogonia/spermatocytes while 20β-HSD was undetectable in haploid cells (spermatids/sperm). These results together with high expression during spawning phase of testicular cycle and after hCG treatment in the prespawning catfish suggests a pivotal role for 20β-HSD during testicular recrudescence leading to sperm maturation. Further studies using various fish models on testicular 20β-HSD may provide interesting details to understand its importance in teleostean spermatogenesis. © 2011 Elsevier Inc.