Identification of RAPD markers associated with Helminthosporium leaf blight (HLB) disease resistance in wheat

Abstract

To detect a set of RAPD markers associated with spot blotch/ Helminthosporium leaf blight (HLB) resistance in wheat, F 2 population of a cross, WH 147 x Chirya 3, comprising 332 individuals was analyzed by Bulked segregant analysis (BSA). Four putative polymorphic and reproducible RAPD markers; OPK06 791, OPA10 1040, OPN15 765 and OPA12 464 were identified. The markers, OPK06 791 and OPA10 1040 were associated with resistance in repulsion phase while, OPN15 765 and OPA12 464 in coupling phase. Together, these markers determined a significant proportion of total phenotypic variation (25.05%). Regression analysis revealed three markers, OPK06 791, OPA10 1040 and OPN15 765 to be significantly associated with disease phenotype.