Animal Biology - Theses

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https://dspace.uohyd.ac.in/handle/1/116

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Now showing 1 - 5 of 122
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    Understanding the role of PAX6 in Corneal Epithelial Regulation at the Limbal Niche
    (University of Hyderabad, 2022-02-16) Vinay Kumar, P ; Indumathi Mariappan
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    Regulatory interplay between chromatin organization and lineage determinants during B cell specification
    (University of Hyderabad, 2022-06-22) Anurupa Devi Yadavalli ; Jagan Pongubala
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    Spatiotemporal Dynamics of Chromatin Condensation During Embryonic Stem Cells to Neuronal Differentiation
    (University of Hyderabad, 2022-03-09) Prashanth Kumar, G ; Sreenivasulu Kurukuti
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    Multi-OMICS Approach Towards Understanding the Pathogenesis of Retinopathy of Prematurity
    (University of Hyderabad, 2021-11) Satish Patnaik, B. ; Kaur, Inderjeet ; Madhava Reddy, Bindu
    Retinopathy of prematurity (ROP) is a complex vaso-proliferative eye condition that affects prematurely born infants worldwide. It has a high prevalence of 24-47% in India. Despite timely interventions, a proportion of babies progress to severe stages of the disease. The overall molecular etiology leading to ROP pathogenesis is not well known.
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    Toxic effects of allethrin on the male reproductive system
    (University of Hyderabad, 2014) Madhu Babu, Golla ; Suresh, Y
    Over the past decades, there has been an increased concern about the impact of environmental contaminants like pesticides on human reproductive system. These pesticides are used for both agricultural and non-agricultural purposes throughout the world. Synthetic pyrethroids persist in the environment and can enter into the geological cycle which can influence reproductive health. Allethrin, the first synthetic pyrethroid, which knock down insects has neurological effects and no reports are available about the impact on male reproductive system. In the present study, we studied the toxic effects of allethrin on male reproductive system using rat as a model system. We identified the molecular mechanism of allethrin cytotoxicity in rat Leydig cell carcinoma cells (LC540). Allethrin shows cytotoxicity in a concentration dependent manner. It increases lipid peroxidation and alters antioxidant enzyme status. Morphological analyses of LC540 cells treated with allethrin revealed the presence of apoptotic bodies. Using flow cytometry, we observed that the number of cells that displayed early apoptotic features and entering into G0 phase of cell cycle increased significantly with loss of mitochondrial membrane potential. The levels of p53 mRNA and cleaved PARP-1 protein were increased, whereas BCL-2, pro-Caspase-3 and PARP- 1 were decreased. Allethrin induced apoptosis was associated with voltage gated calcium channel mediated intracellular calcium release. Besides cytotoxicity, the reduced expression of spermatogenic factors namely Tgf-β1 and Scf suggests the potential of allethrin to affect spermatogenesis