Protein stiffening and entropic stabilization in the subdenaturing limit of guanidine hydrochloride.
Protein stiffening and entropic stabilization in the subdenaturing limit of guanidine hydrochloride.
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Date
2004-01-01
Authors
Kumar, Rajesh
Prabhu, N. Prakash
Yadaiah, M.
Bhuyan, Abani K.
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Abstract
Subdenaturing concentrations of guanidine hydrochloride (GdnHCl) stabilize proteins. For ferrocytochrome c the stabilization is detected at subglobal level with no measured change in global stability. These deductions are made by comparing observed rates of thermally driven ferrocytochrome cHCO reactions with global unfolding rates of ferrocytochrome c measured by stopped flow and NMR hydrogen exchange in the presence of a wide range of GdnHCl concentrations at pH 7, 22 degrees C. Copyright 2004 Biophysical Society
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Biophysical journal. v.87(4)