Roseomonas oryzae sp. nov., isolated from paddy rhizosphere soil

Abstract

A non-motile, coccus-shaped, pale-pink-pigmented bacterium, designated strain JC288T, was isolated from a paddy rhizosphere soil collected from Western Ghats, Kankumbi, Karnataka, India. Cells were found to be Gram-stain-negative, and catalase- and oxidase-positive; the major fatty acids were C16: 0,C16: 1ω7c/C16: 1ω6c,C18: 1ω7c/C18: 1ω6c and C18: 12-OH. The predominant respiratory quinone was Q-10 and the genomic DNA G + C content was 67.5 mol%. Strain JC288T contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, four unidentified aminolipids, three unidentified phospholipids, two unidentified lipids, an aminophospholipid and a glycolipid. Hydroxyspirilloxanthin was the major carotenoid of strain JC288T. 16S rRNA gene sequence comparisons indicated that strain JC288T represents a member of the genus Roseomonas within the family Acetobacteraceae of the phylum Proteobacteria. Strain JC288T shared the highest 16S rRNA gene sequence similarity with Roseomonas rhizosphaerae YW11T (97.3%), Roseomonas aestuarii JC17T (97.1%), Roseomonas cervicalis CIP 104027T (95.9%) and other members of the genus Roseomonas ( < 95.5%). The distinct genomic difference and morphological, physiological and chemotaxonomic differences from the previously described taxa support the classification of strain JC288T as a representative of a novel species of the genus Roseomonas, for which the name Roseomonas oryzae sp. nov. is proposed. The type strain is JC288T (=KCTC 42542T=LMG 2871 1T).