Salinimicrobium sediminis sp. nov., isolated from a deep-sea sediment

Abstract

Strain JC207T was isolated from a deep (265 m) sea sediment, and appeared as dark yellow colonies on agar plates with cells staining Gram-negative. Catalase, oxidase and caseinase were positive, while chitinase, gelatinase and amylase were negative. Major ( > 5 %) fatty acids were iso-C15: 0, anteiso-C15: 0, iso-C17: 1ω9c, iso-C16: 0, iso-C15: 0 3-OH, iso-C17: 0 3-OH, iso-C14: 0 and iso-C15: 1G. Strain JC207T contained phosphatidylethanolamine as the major polar lipid, with minor amounts of five unidentified lipids. A bacterial hopane derivative, diplopterol and adenosylhopane were the major hopanoids. Genomic DNA G+C content was 47.5 mol%. 16S rRNA gene sequence comparisons indicated that strain JC207T represented a member of the genus Salinimicrobium within the family Flavobacteriaceae of the phylum Bacteroidetes. Strain JC207T had sequence similarity withSalinimicrobium terrae YIM-C338T (98 %), Salinimicrobium xinjiangenseBH206T (97.6 %) and other members of the genus Salinimicrobium ( < 96.8 %). However, strain JC207T showed an average of 23.6±4 and 37±4 relatedness (based on DNA-DNA hybridization) with Salinimicrobium terraeCGMCC 1.6308T (= YIM-C338T) and Salinimicrobium xinjiangense KCTC 12883T (= BH206T), respectively. Morphological, physiological and genotypic differences from the previously described taxa support the classification of strain JC207T as a representative of a novel species in the genusSalinimicrobium, for which the name Salinimicrobium sediminis sp. nov. is proposed. The type strain is JC207T (= KCTC 32444T = CGMCC 1.12641T). © 2014 IUMS.