Rapid detection of fungal filaments in corneal scrapings by microwave heating-assisted Grocott's methenamine silver staining

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Date
2002-12-01
Authors
Vemuganti, Geeta K.
Naidu, Chenchu
Gopinathan, Usha
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Abstract
The Gomoris methanamine silver impregnation technique is a highly reliable and archivable method of detecting fungal filaments, but the staining procedure is time consuming and laborious. A technique using microwave energy to reduce the duration of Gomori's silver staining is described.
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Keywords
Corneal scrapings, Fungus, Giemsa stain, Gram's stain, KOH staon/Calcofluor stain, Microwave heating
Citation
Indian Journal of Ophthalmology. v.50(4)