BpaI and BpnI: Novel type II restriction endonucleases from Bacillus pasteurii and Bacillus pantothenticus
BpaI and BpnI: Novel type II restriction endonucleases from Bacillus pasteurii and Bacillus pantothenticus
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Date
2004-06-01
Authors
Jutur, Pavan P.
Reddy, Attipalli R.
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Abstract
Two novel type II restriction endonucleases, designated as BpaI and BpnI, were isolated from Bacillus pasteurii strain1761 and Bacillus pantothenticus strain1639, respectively. They were partially purified and SDS-PAGE indicated Mr values of 28 and 67 kDa for BpaI, 28 and 48 kDa for BpnI. The partially purified endonucleases hydrolyzed DNA into discrete fragments: pUC18 (2.6 kb for BpaI; 1.8 and 0.8 kb for BpnI), pBR322 (2.5 and 1.8 kb for BpaI; 2.6 and 1.7 kb for BpnI) and Φx174 DNA (3.2 and 2.1 kb for BpaI; 4 and 1.3 kb for BpnI).
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Keywords
Bacillus pantothenticus,
Bacillus pasteurii,
Restriction endonuclease
Citation
Biotechnology Letters. v.26(11)