BpaI and BpnI: Novel type II restriction endonucleases from Bacillus pasteurii and Bacillus pantothenticus

dc.contributor.author	Jutur, Pavan P.
dc.contributor.author	Reddy, Attipalli R.
dc.date.accessioned	2022-03-27T03:45:06Z
dc.date.available	2022-03-27T03:45:06Z
dc.date.issued	2004-06-01
dc.description.abstract	Two novel type II restriction endonucleases, designated as BpaI and BpnI, were isolated from Bacillus pasteurii strain1761 and Bacillus pantothenticus strain1639, respectively. They were partially purified and SDS-PAGE indicated Mr values of 28 and 67 kDa for BpaI, 28 and 48 kDa for BpnI. The partially purified endonucleases hydrolyzed DNA into discrete fragments: pUC18 (2.6 kb for BpaI; 1.8 and 0.8 kb for BpnI), pBR322 (2.5 and 1.8 kb for BpaI; 2.6 and 1.7 kb for BpnI) and Φx174 DNA (3.2 and 2.1 kb for BpaI; 4 and 1.3 kb for BpnI).
dc.identifier.citation	Biotechnology Letters. v.26(11)
dc.identifier.issn	01415492
dc.identifier.uri	10.1023/B:bile.0000025905.14101.39
dc.identifier.uri	http://link.springer.com/10.1023/B:bile.0000025905.14101.39
dc.identifier.uri	https://dspace.uohyd.ac.in/handle/1/5129
dc.subject	Bacillus pantothenticus
dc.subject	Bacillus pasteurii
dc.subject	Restriction endonuclease
dc.title	BpaI and BpnI: Novel type II restriction endonucleases from Bacillus pasteurii and Bacillus pantothenticus
dc.type	Journal. Article
dspace.entity.type

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